Tag
Salty_D20

Part:BBa_K562001:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)


Salty_PduD20 Targeting Tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 179
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 179
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fragment carries an XbaI site at the extreme 3' end. Thus, to clone your gene of interest in-frame with this targeting sequence all you need do is place the 6-nucleotide XbaI site immediately upstream of your ATG initiation codon.


Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.

References